High Performance Thin Layer Chromatography has been developed for simultaneous estimation of Diazepam and Imipramine Hydrochloride. The method involved measurement of absorbance difference at 274.0 nm The separation was carried out on Merck HPTLC aluminum sheets of silica gel 60F254 using Toluen:Methanol:FormicAcid (8:2.0:0.2 v/v/v/v) as mobile phase. The linear regression analysis data was used for the regression line in the range of 300–800 ng/spot and 2250–6000 ng/spot for DIZ and IMP, respectively. The percent assay of marketed formulation was 100.10±0.12 and 99.96±0.26 of Diazepam and Imipramine Hydrochloride respectively. The proposed methods are rapid, simple, accurate and precise since they are recommended for routine analysis.
Loading....